Gene Symbol | Mocs3 |
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Entrez Gene | 69372 |
Alt Symbol | 1700020H17Rik, Uba4 |
Species | Mouse |
Gene Type | protein-coding |
Description | molybdenum cofactor synthesis 3 |
Other Description | MPT synthase sulfurylase|adenylyltransferase and sulfurtransferase MOCS3|molybdopterin synthase sulfurylase |
Swissprots | A2BDX3 |
Accessions | A2BDX3 EDL06550 AK006163 BC115985 AAI15986 BE133066 BE333092 BG083535 BY727851 NM_001160330 NP_001153802 |
Function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. {ECO:0000255|HAMAP-Rule:MF_03049}. |
Subcellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03049}. |
Top Pathways | Sulfur relay system |
MOCS3 CRISPR/Cas9 KO Plasmid (m) - sc-427395 from Santa Cruz Biotechnology
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MOCS3 HDR Plasmid (m) - sc-427395-HDR from Santa Cruz Biotechnology
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MOCS3 Double Nickase Plasmid (m) - sc-427395-NIC from Santa Cruz Biotechnology
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MOCS3 Double Nickase Plasmid (m2) - sc-427395-NIC-2 from Santa Cruz Biotechnology
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MOCS3 CRISPR Activation Plasmid (m) - sc-427395-ACT from Santa Cruz Biotechnology
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MOCS3 CRISPR Activation Plasmid (m2) - sc-427395-ACT-2 from Santa Cruz Biotechnology
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MOCS3 Lentiviral Activation Particles (m) - sc-427395-LAC from Santa Cruz Biotechnology
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MOCS3 Lentiviral Activation Particles (m2) - sc-427395-LAC-2 from Santa Cruz Biotechnology
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MOCS3 siRNA (m) - sc-149496 from Santa Cruz Biotechnology
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MOCS3 shRNA Plasmid (m) - sc-149496-SH from Santa Cruz Biotechnology
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MOCS3 shRNA (m) Lentiviral Particles - sc-149496-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN35686 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND35686 from Sigma-Aldrich
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MOCS3 siRNA (Mouse) - CRM7956 from Cohesion Biosciences
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shRNA set against Mouse MOCS3 (NM_001160330.1) - SHH343396 from Creative biogene
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shRNA set against Mouse Mocs3(NM_001160330.1) - SHH195239 from Creative biogene
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Mouse MOCS3 siRNA - orb287432 from Biorbyt
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MOCS3 shRNA Plasmid (Mouse) - CHM7956 from Cohesion Biosciences
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MOCS3 miRNA 3'UTR clone - MiUTR1M-07202 from Creative biogene
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