B/N Blocking/neutralizing



Anti-67kDa Laminin Receptor antibody [MLuC5] - ab3099 from Abcam

Prices $398.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone MLuC5
ICC/IF image of ab3099 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3099, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-67kDa Laminin Receptor antibody [MLuC5] - ab80582 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone MLuC5
Formalin-fixed, paraffin-embedded human breast carcinoma stained with ab80582 at 8µg/ml using a peroxidase-conjugate and DAB chromogen. Note cell membrane with some cytoplasmic staining of tumor cells.

Anti-Acetylcholinesterase antibody [ZR3] - ab2802 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ZR3
Immunofluorescent analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) ab2802 shows staining in C6 glioma cells. Acetylcholinesterase staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase ab2802 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Anti-Activin antibody [MM0074-7L18] - ab89307 from Abcam

Prices $380.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM0074-7L18
All lanes : Anti-Activin antibody [MM0074-7L18] (ab89307) at 1 µg/mlLane 1 : Embryonic Day 18 LiverLane 2 : Embryonic Day 18 MusclesLane 3 : Embryonic Day 18 Cortex (Negative Control)developed using the ECL techniquePerformed under reducing conditions.

Anti-Activin Receptor Type IIA antibody - ab10595 from Abcam

Prices $372.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-Activin Receptor Type IIB antibody - ab10596 from Abcam

Prices $378.00
Sizes 25 µg
Host Goat
Clonality Polyclonal
ACVR2B staining was mainly localized at the membrane level of the epithelial cells in the normal tissues with a clear down-regulation in tumoral epithelia.

Anti-ADAMTS1 antibody - Aminoterminal end - ab28284 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ADAMTS1 antibody - Aminoterminal end (ab28284) at 1/2500 dilution + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-ADAMTS4 antibody [MM0081-10D5] - ab89112 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone MM0081-10D5

Anti-ADAMTS9 antibody - ab28279 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Adenovirus Type 5 antibody - ab6982 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.

Anti-Adiponectin antibody - ab3455 from Abcam

Prices $384.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal
Western blot detection of Adiponectin on mouse serum using ab3455.

Anti-alpha 1 Sodium Potassium ATPase antibody [9-A5] - ab2867 from Abcam

Prices $392.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9-A5
Immunocytochemistry/Immunofluorescence analysis of alpha 1 Sodium Potassium ATPase shows staining in HeLa cells. alpha 1 Sodium Potassium ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2867 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] - ab2872 from Abcam

Prices $404.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone M8-P1-A3
Immunocytochemistry/Immunofluorescence analysis of alpha 1 Sodium Potassium ATPase (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were incubated with ab2872 (1:100) for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (1:500) for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10] - ab2826 from Abcam

Prices $380.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone XVIF9-G10
Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10] (ab2826) at 1/1000 dilution (5% Milk for 1 hour 30 minutes at 24°C.) + Human brain cortex whole tissue lysate (10µg). with 5% Milk for 1 hour for 24°C.SecondaryAn HRP-conjugated goat anti-mouse IgG1 at 1/5000 dilutionThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-alpha A Crystallin antibody - ab5595 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab5595 staining alpha A Crystallin in non-transgenic 13.5 mouse embryos by Immunohistochemistry (PFA fixed, paraffin embedded sections).Heads of mice were removed, fixed in 10% formalin, dehydrated, embedded in paraffin, sectioned (5-7 µm).Slides containing ocular sections from non-transgenic embryos were first deparaffinized and rehydrated. Antigens were retrieved by microwave treatment in 10 mM Sodium Citrate buffer (pH 6.0). Following antigen retrieval, the tissue sections were blocked with 10% normal horse serum for 30 minutes, at room temperature. The slides were then incubated with ab5595 at a 1/10,000 dilution overnight at 4°C. Following brief washes in PBS, the slides were incubated with the appropriate biotinylated-secondary antibodies for 30 minutes at 37°C. Antigen-antibody complexes were then detected using streptavidin-linked Alexa 594 (red) at 1/1000 dilution. Sections were mounted using anti-fade media containing DAPI (blue). Images were captured using a Nikon Eclipse E6

Anti-alpha A Crystallin antibody [c9F2] - ab14821 from Abcam

Prices $373.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone c9F2

Anti-alpha Adaptin antibody [AP6] - ab2730 from Abcam

Prices $403.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AP6
Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in U251 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Anti-alpha Adaptin antibody [AP6] - ab2730 from Abcam

Prices $403.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AP6
Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in U251 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Anti-Alpha Dystroglycan antibody [4A145] - ab64568 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4A145

Anti-alpha-hemolysin antibody [8B7] - N-terminal - ab190467 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 8B7
Toxin neutralization: Using a rabbit RBC lysis assay, EC50 of ab190467 for neutralization of 0.3 μg/mL of alpha-hemolysin was determined to be 0.676 μg/mL.

Anti-Antithrombin III antibody [BDI205] - ab20933 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BDI205
IHC image of Antithrombin III staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20933, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-APE1 antibody - ab82 from Abcam

Prices $373.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP1A2 antibody [M7-PB-E9] - ab2871 from Abcam

Prices $370.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone M7-PB-E9
ATP1A2 was immunoprecipitated using 0.5mg Mouse Kidney tissue extract, 5µg of Mouse monoclonal to ATP1A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2871.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 102kDa; ATP1A2

Anti-B7 DC antibody [TY25] - ab21107 from Abcam

Prices $378.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone TY25

Anti-BCA1 antibody [MM0212-12A2] - ab89322 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone MM0212-12A2
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