RPS3

Gene Symbol	RPS3
Entrez Gene	6188
Alt Symbol	S3
Species	Human
Gene Type	protein-coding
Description	ribosomal protein S3
Other Description	40S ribosomal protein S3\|IMR-90 ribosomal protein S3
Swissprots	Q498B5 Q8NI95 J3KN86 B2R7N5 P23396
Accessions	AAA18095 AAF82383 AAV40835 BAB79476 EAW74963 P23396 AA976344 AB062288 BAB93471 AK129794 AK130960 AK223048 BAD96768 AK313051 BAG35882 AW134501 BC003137 AAH03137 BC003577 AAH03577 BC013196 AAH13196 BC013231 BC029981 BC034149 AAH34149 BC071669 BC071917 AAH71917 BC100284 AAI00285 BC150501 AAI50502 BE797417 BM831460 BM928330 BQ645330 CB241053 D28344 BAA05710 DQ892888 ABM83814 DQ896136 ABM87135 DW448503 S42658 AAB19349 U14990 AAB60336 U14991 AAB60337 U14992 AAB60338 X55715 CAA39248 NM_001005 NP_000996 NM_001256802 NP_001243731 NM_001260506 NP_001247435 NM_001260507 NP_001247436
Function	Involved in translation as a component of the 40S small ribosomal subunit (PubMed:8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571). Enhances the uracil excision activity of UNG1 (PubMed:18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537). Has also been shown to negatively regulate DNA repair in
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:16314389, ECO:0000269\|PubMed:17289661, ECO:0000269\|PubMed:17560175, ECO:0000269\|PubMed:18045535, ECO:0000269\|PubMed:20217897, ECO:0000269\|PubMed:21871177}. Nucleus {ECO:0000269\|PubMed:17560175, ECO:0000269\|PubMed:18045535, ECO:0000269\|PubMed:19460357, ECO:0000269\|PubMed:20217897, ECO:0000269\|PubMed:21871177}. Nucleus, nucleolus {ECO:0000269\|PubMed:16314389, ECO:0000269\|PubMed:19460357}. Mitochondrion inner membrane {ECO:0000269\|PubMed:23911537}; Peripheral membrane protein {ECO:0000269\|PubMed:23911537}. Cytoplasm, cytoskeleton, spindle {ECO:0000269\|PubMed:23131551}. Note=In normal cells, located mainly in the cytoplasm with small amounts in the nucleus but translocates to the nucleus in cells undergoing apoptosis (By similarity). Nuclear translocation is induced by DNA damaging agents such as hydrogen peroxide (PubMed:17560175). Accumulates in the mitochondrion in response to increased ROS levels (PubMed:23911537). Localizes to the spindle during mitosis (
Top Pathways	Ribosome

Ribosomal Protein S3 (D50G7) XP ® Rabbit mAb - 9538 from Cell Signaling Technology

Prices	$109.00, $277.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D50G7

Western blot analysis of extracts from various cell types using Ribosomal Protein S3 (D50G7) XP ® Rabbit mAb.

Ribosomal Protein S3 Antibody - 2579 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from various cell types using Ribosomal Protein S3 Antibody.

Ribosomal Protein S3 (C-7) - sc-376008 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Ribosomal Protein S3 (C-7): sc-376008. Immunoperoxi-dase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing cytoplasmic staining of respiratory epithelial cells.

Ribosomal Protein S3 (E-6) - sc-376098 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Ribosomal Protein S3: sc-376098. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

Ribosomal Protein S3 (FL-243) - sc-135390 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Ribosomal Protein S3 (FL-243): sc-135390. Western blot analysis of Ribosomal Protein S3 expression in HeLa (A) and Raji (B) whole cell lysates.

Ribosomal Protein S3 (P-12) - sc-169173 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Ribosomal Protein S3 (P-12): sc-169173. Western blot analysis of Ribosomal Protein S3 expression in HeLa whole cell lysate.

Anti-RPS3 antibody - ab77772 from Abcam

Prices	$384.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-RPS3 antibody (ab77772) at 1 µg/mlLane 1 : Liver (Mouse) Tissue Lysate Lane 2 : Kidney (Mouse) Tissue Lysate Lane 3 : Liver (Rat) Tissue Lysate Lane 4 : Lung (Rat) Tissue Lysate Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-RPS3 antibody - ab140676 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-RPS3 antibody (ab140676) at 0.1 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-RPS3 antibody - ab140688 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Detection of RPS3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140688 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-RPS3 antibody - ab154953 from Abcam

Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RPS3 antibody (ab154953) at 1/1000 dilution + Raji whole cell lysate at 30 µg

Anti-RPS3 antibody - ab77330 from Abcam

Prices	$378.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-RPS3 antibody (ab77330) at 5 µg/ml + immunogen at 0.2 µg/mlSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilutiondeveloped using the ECL technique

Anti-RPS3 antibody - ab125372 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RPS3 antibody (ab125372) at 1 µg/ml + Human fetal kidney lysate at 10 µg

Anti-RPS3 antibody [EPR7807] - ab181992 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7807

All lanes : Anti-RPS3 antibody [EPR7807] (ab181992) at 1/2000 dilutionLane 1 : HepG2 cell lineLane 2 : Raw264.7 cell lineLane 3 : NIH 3T3 cell lineLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-RPS3 antibody [EPR7808] - Ribosome Marker - ab128995 from Abcam

Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7808

All lanes : Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : PC-12 cell lysateLane 3 : Neuro-2a cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-RPS3 / Ribosomal Protein S3 Antibody - LS-C75466 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RPS3 / Ribosomal Protein S3 Antibody (aa116-165) - LS-C151544 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

S3 / RPS3 antibody Western Blot of A549.

Anti-S3 / RPS3 Antibody (aa144-244, Azide-free) - LS-C328536 from LifeSpan Bioscience

Host	Mouse
Clonality	Monoclonal

Anti-RPS3 / Ribosomal Protein S3 Antibody (aa198-243) - LS-C313932 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RPS3 / Ribosomal Protein S3 Antibody - LS-C341431 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RPS3 / Ribosomal Protein S3 Antibody (C-Terminus) - LS-C146497 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

RPS3 antibody LS-C146497 Western blot of Fetal Kidney lysate. Antibody concentration 1 ug/ml.

Anti-RPS3 / Ribosomal Protein S3 Antibody (aa160-209) - LS-C200625 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of the lysates from RAW264.7cells using RPS3 antibody.

Anti-S3 / RPS3 Antibody (aa193-243) - LS-C290490 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human RPS3 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RPS3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-S3 / RPS3 Antibody (aa1-50) - LS-C290488 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RPS3 / Ribosomal Protein S3 Antibody (aa171-220) - LS-C119646 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of paraffin-embedded human tonsil tissue, using RPS3 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-RPS3 / Ribosomal Protein S3 Antibody (clone 2A8) - LS-C198118 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2A8

RPS3 monoclonal antibody (M03), clone 2A8. Western Blot analysis of RPS3 expression in HeLa NE.

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