AAK1
| Gene Symbol | AAK1 |
|---|---|
| Entrez Gene | 22848 |
| Alt Symbol | - |
| Species | Human |
| Gene Type | protein-coding |
| Description | AP2 associated kinase 1 |
| Other Description | AP2-associated protein kinase 1|adaptor-associated kinase 1 |
| Swissprots | Q4ZFZ3 Q2M2I8 Q9UPV4 Q53RX6 |
| Accessions | AAX88861 AAY14931 EAW99847 EAW99848 Q2M2I8 AB028971 BAA83000 AF090100 AF090101 AK055622 AK056043 AK074292 AK098797 AK124872 AK127187 BAC86877 AK310951 AW451954 AW969393 BC002695 AAH02695 BC073953 BC090950 BC104842 AAI04843 BC111965 AAI11966 BC132937 BC132939 BC143710 AAI43711 BX640963 BX641060 CA436375 DA121999 DB285703 NM_014911 NP_055726 |
| Function | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. {ECO:0000269|PubMed:12952931, ECO:0000269|PubMed:17494869, ECO:0000269|PubMed:18657069, ECO:0000269|PubMed:21464124}. |
| Subcellular Location | Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Membrane, clathrin-coated pit {ECO:0000250}. Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity). {ECO:0000250}. |
| Tissue Specificity | Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain. {ECO:0000269|PubMed:17494869}. |
AAK1 CRISPR/Cas9 KO Plasmid (h) - sc-406073 from Santa Cruz Biotechnology
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AAK1 HDR Plasmid (h) - sc-406073-HDR from Santa Cruz Biotechnology
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AAK1 Double Nickase Plasmid (h) - sc-406073-NIC from Santa Cruz Biotechnology
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AAK1 Double Nickase Plasmid (h2) - sc-406073-NIC-2 from Santa Cruz Biotechnology
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AAK1 CRISPR Activation Plasmid (h) - sc-406073-ACT from Santa Cruz Biotechnology
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AAK1 CRISPR Activation Plasmid (h2) - sc-406073-ACT-2 from Santa Cruz Biotechnology
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AAK1 Lentiviral Activation Particles (h) - sc-406073-LAC from Santa Cruz Biotechnology
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AAK1 Lentiviral Activation Particles (h2) - sc-406073-LAC-2 from Santa Cruz Biotechnology
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AAK1 siRNA (h) - sc-94850 from Santa Cruz Biotechnology
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AAK1 shRNA Plasmid (h) - sc-94850-SH from Santa Cruz Biotechnology
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AAK1 shRNA (h) Lentiviral Particles - sc-94850-V from Santa Cruz Biotechnology
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AAK1 RNAi - H00022848-R02 from Novus Biologicals
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MISSION® esiRNA - EHU084031 from Sigma-Aldrich
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MISSION® siRNA - SIHK0002 from Sigma-Aldrich
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MISSION® siRNA - SIHK0001 from Sigma-Aldrich
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MISSION® siRNA - SIHK0003 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN2608 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2608 from Sigma-Aldrich
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AAK1 siRNA (Human) - CRH7810 from Cohesion Biosciences
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AAK1 - MBS8211626 from MyBioSource
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Human AAK1 siRNA - orb268312 from Biorbyt
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AAK1 shRNA Plasmid (Human) - CHH7810 from Cohesion Biosciences
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