EIF2S1

Gene Symbol EIF2S1
Entrez Gene 1965
Alt Symbol EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Other Description eIF-2-alpha|eukaryotic translation initiation factor 2 subunit 1|eukaryotic translation initiation factor 2 subunit alpha
Swissprots P05198
Accessions EAW80933 EAW80934 P05198 AK297244 BAG59724 AK312442 BAG35351 BC002513 AAH02513 BX161521 CAD61953 D28379 BAA05745 DQ892151 ABM83077 DQ895347 ABM86273 J02645 AAA52373 U26032 NM_004094 NP_004085
Function Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Subcellular Location Cytoplasmic granule {ECO:0000250}. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). {ECO:0000250}.
Top Pathways Protein processing in endoplasmic reticulum, RNA transport, Hepatitis C, Measles, Influenza A

Phospho-eIF2α (Ser51) (D9G8) XP ® Rabbit mAb (Biotinylated) - 5199 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9G8
Western blot analysis of extracts from NIH/3T3 cells, untreated or thapsigargin-treated, using Phospho-eIF2α (Ser51) (D9G8) XP ® Rabbit mAb (Biotinylated).

eIF2α (D7D3) XP ® Rabbit mAb (Biotinylated) - 9079 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7D3
Western blot analysis of extracts from HeLa and NIH/3T3 cells using eIF2α (D7D3) XP ® Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for detection.

Phospho-eIF2α (Ser51) (D9G8) XP ® Rabbit mAb - 3398 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9G8
Western blot analysis of extracts from C2C12 cells, untreated or thapsigargin-treated, using Phospho-eIF2α (Ser51) (D9G8) XP ® Rabbit mAb (upper) or eIF2α Antibody #9722 (lower).

Phospho-eIF2α (Ser51) (119A11) Rabbit mAb - 3597 from Cell Signaling Technology

Prices $307.00, $719.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 119A11
Western blot analysis of extracts from HT29 (human) and AR42J (mouse) cells, untreated or thapsigargin-treated (300 nM, 30 min), using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb (upper) or eIF2alpha Antibody #9722 (lower).

Phospho-eIF2α (Ser51) Antibody - 9721 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM), using Phospho-eIF2alpha (Ser51) Antibody (upper) or eIF2alpha Antibody #9722 (lower).

eIF2α (D7D3) XP ® Rabbit mAb - 5324 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7D3
Western blot analysis of extracts from various cell lines using eIF2α (D7D3) XP ® Rabbit mAb.

eIF2α (L57A5) Mouse mAb - 2103 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L57A5
Western blot analysis of extracts from various cell types using eIF2α (L57A5) Mouse mAb.

eIF2α Antibody - 9722 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM) using Phospho-eIF2α (Ser51) Antibody #9721 (upper) and control eIF2α Antibody (lower).

eIF2α (D-3) - sc-133132 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
eIF2α (D-3): sc-133132. Western blot analysis of eIF2α expression in HeLa (A), K-562 (B), A-431 (C) and KNRK (D) whole cell lysates.

eIF2α (FL-315) - sc-11386 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
eIF2α (FL-315): sc-11386. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

eIF2α (K-17) - sc-30882 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF2α (K-17): sc-30882. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

eIF2α (G-12) - sc-133227 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
eIF2α (G-12): sc-133227. Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.

p-eIF2α (Ser 49) - sc-293100 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-eIF2α (Ser 49): sc-293100. Western blot analysis of eIF2α phosphorylation expression in untreated (A) and serum-starved (B) HeLa whole cell lysates.

p-eIF2α (Ser 52) - sc-12412 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-eIF2α (Ser 52): sc-12412. Western blot analysis of eIF2α phosphorylation in HeLa whole cell lysate.

p-eIF2α (Ser 52) - sc-101670 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-eIF2α (Ser 52): sc-101670. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.

Anti-EIF2S1 (phospho S49) antibody - ab131489 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of methanol-fixed HeLa cells labelling EIF2S1 using ab131489, at 1/100 dilution.

Anti-EIF2S1 (phospho S51) antibody - ab4837 from Abcam

Prices $400.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF2S1 (phospho S51) antibody - ab131505 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131505 at 1/100 dilution.

Anti-EIF2S1 (phospho S51) antibody - ab47769 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EIF2S1 (phospho S51) antibody (ab47769) at 1/500 dilutionLane 1 : K562 cell extract, untreated.Lane 2 : K562 cell extract treated with IFN alpha (1000U/ml, 18 hours)

Anti-EIF2S1 (phospho S51) antibody [E90] (Alexa Fluor® 488) - ab196460 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E90
Overlay histogram showing A431 cells stained with ab196460 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196460, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-EIF2S1 (phospho S51) antibody [E90] (Alexa Fluor® 647) - ab196191 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E90
ab196191 staining EIF2S1 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab196191 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-EIF2S1 antibody - ab131495 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EIF2S1 antibody (ab131495) at 1/500 dilutionLane 1 : 3T3 cell extractLane 2 : HeLa cell extractLane 3 : C6 cell extract

Anti-EIF2S1 antibody - ab137626 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-EIF2S1 antibody (ab137626) at 1/500 dilution + H1299 whole cells lysate at 30 µg

Anti-EIF2S1 antibody - ab65744 from Abcam

Prices $376.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal
Anti-EIF2S1 antibody (ab65744) at 2 µg/ml + HEK293 cell extracts

Anti-EIF2S1 antibody - ab47508 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab47508 at 1/50 dilution staining paraffin-embedded human breast carcinoma tissue; Left without and Right with immunising peptide (1ug/ml).
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