NOS3

Gene Symbol NOS3
Entrez Gene 287024
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description nitric oxide synthase 3 (endothelial cell)
Other Description EC-NOS|NOS type III|NOSIII|cNOS|constitutive NOS|eNOS|endothelial NOS|endothelial nitric oxide synthase|nitric oxide synthase, endothelial
Swissprots P29473
Accessions CAA02941 DAA30266 P29473 M89952 AAA30494 M95674 AAA30669 M99057 AAA30667 NM_181037 NP_851380
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Subcellular Location Cell membrane {ECO:0000269|PubMed:17071725}. Membrane, caveola {ECO:0000269|PubMed:17071725}. Cytoplasm, cytoskeleton {ECO:0000250}. Golgi apparatus {ECO:0000269|PubMed:17071725}. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. {ECO:0000250}.
Top Pathways Sphingolipid signaling pathway, cGMP-PKG signaling pathway, Oxytocin signaling pathway, Estrogen signaling pathway, PI3K-Akt signaling pathway

Anti-eNOS (phospho S1177) antibody - ab75639 from Abcam

Host Rabbit
Clonality Polyclonal
ab75639 staining eNOS (phospho S1177) in Bovine aortic endothelial cells ( by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% saponin and blocked with PBS + 5% BSA + 0.1% saponin for 1 hour at 37°C. Samples were incubated with primary antibody (1/150 in PBS + 1% BSA + 0.1% saponin) for 16 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

Anti-eNOS (phospho S1177) antibody - ab87750 from Abcam

Prices $378.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
IHC image of eNOS staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87750, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-eNOS antibody - ab66127 from Abcam

Prices $401.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
The tissue lysate derived from mouse lung was immunoprecipitated with 2µg/ml ab66127, then immuno-blotted by the same antibody at 1/500 (lane 1); Lane 2: BSA as negative control.

Anti-eNOS antibody - ab5589 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunolocalization of eNOS in human lung tissue using ab5589.

Anti-eNOS antibody - ab95254 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-eNOS antibody (ab95254) at 1/250 dilution + Rabbit aorta tissue lysate at 40 µgSecondaryHRP-conjugated Donkey anti-rabbit IgG at 1/2500 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-eNOS antibody - ab50260 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-eNOS antibody - ab3868 from Abcam

Prices $379.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Cell lysate from Pulmonary artery endothelial cells were probed by preimmune serum (line 1) or anti-eNOS (ab3868) (Lane 2).

Anti-eNOS antibody - ab119292 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab119292, at 5 µg/ml, staining eNOS in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.

Anti-eNOS antibody - ab23689 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-eNOS antibody [NOS-E1] - ab50010 from Abcam

Prices $372.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone NOS-E1

Anti-eNOS antibody, prediluted - ab15280 from Abcam

Prices $378.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Human placenta stained with ab15280 in IHC paraffin fixed sections.

NOS3 antibody - PAB13610 from Acris Antibodies

Prices $760.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

eNOS Polyclonal Antibody - PA1-037 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunolocalization staining of eNOS in human lung tissue using PA1-037.

eNOS Polyclonal Antibody - PA3-031A from Thermo Fisher Scientific

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of eNOS using eNOS Polyclonal Antibody (Product# PA3-031A ) shows staining in Hela Cells. eNOS (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing eNOS (Product# PA3-031A ) at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

eNOS Polyclonal Antibody - PA5-16887 from Thermo Fisher Scientific

Prices $309.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed, paraffin-embedded human Lung Ca stained with eNOS antibody using peroxidase-conjugate and AEC. Note staining of endothelium of vessel.

Phospho-eNOS (Ser113) Polyclonal Antibody - PA5-17548 from Thermo Fisher Scientific

Prices $309.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of Phospho-eNOS pSer113 in extracts from bovine aortic endothelial cells (BAEC), untreated or treated with lambda phosphatase, using Phospho-eNOS pSer113 polyclonal antibody (Product # PA5-17548) (upper) or eNOS antibody (lower).

Phospho-eNOS (Ser1177) Monoclonal Antibody (H.83.2) - MA5-14957 from Thermo Fisher Scientific

Prices $309.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone H.83.2
Western blot analysis of Phospho-eNOS pSer1177 in extracts from BAEC cells, phosphatase or VEGF-treated using Phospho-eNOS pSer1177 monoclonal antibody (Product # MA5-14957) (upper) or an ENOS polyclonal antibody (lower).

Phospho-eNOS (Ser1177) Polyclonal Antibody - PA5-17917 from Thermo Fisher Scientific

Prices $309.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Phospho-eNOS (Thr495) Polyclonal Antibody - PA5-17706 from Thermo Fisher Scientific

Prices $309.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Phospho-eNOS pThr495 Antibody - A14042 from Thermo Fisher Scientific

Prices $299.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Phospho-eNOS pSer113 Antibody - A14043 from Thermo Fisher Scientific

Prices $299.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

eNOS - MBS642251 from MyBioSource

Prices $395.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Nitric oxide synthase (endothelial) polyclonal antibody - ALX-210-505-R100 from Enzo Life Sciences

Prices $416.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

eNOS Monoclonal Antibody (9D10) - 33-4600 from Thermo Fisher Scientific

Prices $309.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 9D10

Phospho-eNOS (Ser1179) Polyclonal Antibody - 36-9100 from Thermo Fisher Scientific

Prices $304.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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