NOS3

Gene Symbol NOS3
Entrez Gene 4846
Alt Symbol ECNOS, eNOS
Species Human
Gene Type protein-coding
Description nitric oxide synthase 3 (endothelial cell)
Other Description EC-NOS|NOS type III|NOSIII|cNOS|constitutive NOS|endothelial NOS|nitric oxide synthase 3 transcript variant eNOS-delta20|nitric oxide synthase 3 transcript variant eNOS-delta20-21|nitric oxide synthase 3 transcript variant eNOS-delta21|nitric oxide synthase, endothelial
Swissprots Q14251 Q6GSL5 A8KA63 Q14434 A0S0A8 B2RCQ1 P29474 E9PFR2 Q13662 Q9UDC6 A0S0A7 Q548C1
Accessions AAA36365 AAA36373 AAA36374 AAB87083 AAK71989 AAL07497 AAM74944 ABY87544 BAA05652 CAA53950 CDI44688 EAL24494 EAW54069 P29474 AF400594 AAK83389 AI082109 AK223636 BAD97356 AK225827 AK292928 BAF85617 AK300390 BAH13275 AK302686 BAH13779 AK315213 BAG37648 BC063294 AAH63294 BC069465 AAH69465 BU731828 CB987955 DA859819 DQ256129 ABB79838 DQ256130 ABB79839 DQ256131 ABB79840 HQ257992 ADR82746 JX853577 AFX88320 JX853578 AFX88321 JX853579 AFX88322 M93718 AAA36364 M95296 AAA36372 S80791 AAD14336 XM_006716002 XP_006716065 NM_000603 NP_000594 NM_001160109 NP_001153581 NM_001160110 NP_001153582 NM_001160111 NP_001153583
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Subcellular Location Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Tissue Specificity Platelets, placenta, liver and kidney. {ECO:0000269|PubMed:7515611}.
Top Pathways Sphingolipid signaling pathway, cGMP-PKG signaling pathway, Oxytocin signaling pathway, Estrogen signaling pathway, PI3K-Akt signaling pathway

Phospho-eNOS (Thr495) Antibody - 9574 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from porcine aortic endothelial cells (PAECs), 3 days post-confluence, untreated, H2O2-treated (0.3 mM for 30 minutes) or calf intestinal alkaline phosphatase (CIP)-treated as indicated, using Phospho-eNOS (Thr495) Antibody (upper) or control eNOS antibody (lower).

Phospho-eNOS (Ser1177) Antibody - 9571 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from bovine aortic endothelial cells (BAECs), untreated or VEGF-treated (50 ng/ml) for the indicated times, using Phospho-eNOS (Ser1177) Antibody (upper) or control eNOS antibody (lower).

Phospho-eNOS (Ser113) Antibody - 9575 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from bovine aoric endothelial cells (BAEC), untreated or treated with lamda phosphatase, using Phospho-eNOS (Ser113) Antibody (upper) or eNOS antibody (lower).

Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb - 9570 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone C9C3
Western blot analysis of extracts from BAEC cells, phosphatase or VEGF-treated, using Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb (upper) or eNOS Antibody #9572 (lower).

eNOS (6H2) Mouse mAb - 5880 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 6H2
Western blot analysis of extracts from BAEC and HUVEC cells using eNOS (6H2) Mouse mAb.

eNOS Antibody - 9572 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from bovine aortic endothelial cells, untreated or VEGF-treated, and PAE cells, untreated or UV-treated, using eNOS Antibody.

eNOS (49G3) Rabbit mAb - 9586 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 49G3
Western blot analysis of extracts from BAEC and HUVEC cells, using eNOS (49G3) Rabbit mAb.

NOS3 (A-9) - sc-376751 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NOS3 (A-9): sc-376751. Western blot analysis of NOS3 expression in non-transfected: sc-117752 (A) and mouse NOS3 transfected: sc-122097 (B) 293T whole cell lysates.

NOS3 (B-5) - sc-136977 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NOS3 (B-5): sc-136977. Western blot analysis of NOS3 expression in non-transfected: sc-117752 (A) and mouse NOS3 transfected: sc-122097 (B) 293T whole cell lysates.

NOS3 (C-6) - sc-376542 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NOS3 (C-6): sc-376542. Western blot analysis of NOS3 expression in non-transfected: sc-117752 (A) and mouse NOS3 transfected: sc-122097 (B) 293T whole cell lysates.

NOS3 (C-20) - sc-654 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NOS3 (C-20): sc-654. Western blot analysis of NOS3 expression in rat brain extract.

NOS3 (H-159) - sc-8311 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NOS3 (H-159): sc-8311. Western blot analysis of NOS3 expression in mouse brain extract.

NOS3 (N-20) - sc-653 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NOS3 (N-20): sc-653. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

p-NOS3 (pS1177.39) - sc-293032 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-NOS3 (pT495.33) - sc-136519 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p-NOS3 (pT495.33): sc-136519. Western blot analysis of human endothelial cell lysate (A) and recombinant NOS3 protein containing Thr-495 before in vitro phosphorylation (B) and after in vitro phosphorylation (C).

p-NOS3 (Ser 1177) - sc-12972 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-NOS3 (Ser 1177)-R: sc-12972-R. Western blot analysis of NOS3 phosphorylation in non-transfected: sc-117752 (A) and mouse NOS3 transfected: sc-122097 (B) 293T whole cell lysates.

p-NOS3 (Ser 1177)-R - sc-21871-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal

p-NOS3 (Thr 495) - sc-19827 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of NOS3 phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) HUV-EC-C whole cell lysates. Antibodies tested include p-NOS3 (Thr 495)-R: sc-19827-R (A,B) and NOS3 (C-20): sc-654 (C,D).

p-NOS3 (pS633.37) - sc-136198 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-NOS3 (pS633.37): sc-136198. Western blot analysis of NOS3 phosphorylation in human endothelial cells either untreated (A) or treated (B) with lambda phosphatase.

p-NOS3 (15E2) - sc-81510 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-NOS3 (15E2): sc-81510. Western blot analysis of NOS3 phosphorylation in untreated (A) and pervanadate-treated (B) bend 3 whole cell lysates.

Anti-eNOS (phospho S1176) antibody - ab195944 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-eNOS (phospho S1176) antibody (ab195944) at 1/500 dilutionLane 1 : Insulin treated HeLa whole cell lysates with antigen-specific peptideLane 2 : Insulin treated HeLa whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-eNOS (phospho S1177) antibody - ab51038 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-eNOS (phospho S1177) antibody (ab51038) at 1/500 dilutionLane 1 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), with the immunising peptideLane 2 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), without the immunising peptide

Anti-eNOS (phospho S615) antibody - ab138458 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-eNOS (phospho S615) antibody (ab138458) at 1/500 dilution + K562 cell extracts (from cells treated with EGF 40nM for 30 minutes) at 30 µg

Anti-eNOS (phospho S632) antibody [M232] - ab76199 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone M232
All lanes : Anti-eNOS (phospho S632) antibody [M232] (ab76199) at 1/500 dilutionLane 1 : human umbilical vein endothelial cells, untreatedLane 2 : human umbilical vein endothelial cells, treated with lambda phosphatase

Anti-eNOS (phospho T494) antibody - ab138430 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-eNOS (phospho T494) antibody (ab138430) at 1/500 dilution + Jurkat cell extracts
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