EMSA Gel Shift



Anti-6X His tag® antibody [HIS.H8] - ab18184 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone HIS.H8
Immunofluorescence (red) with ab18184 on His-tag fusion protein in 293 cells.Left hand panel: untransfected controlRight hand panel: transfected.Counterstained with DAPI (blue).

Anti-Acetylcholinesterase antibody [ZR3] - ab2802 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ZR3
Immunofluorescent analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) ab2802 shows staining in C6 glioma cells. Acetylcholinesterase staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase ab2802 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Anti-Androgen Receptor antibody [AN1-15] - ab2742 from Abcam

Prices $385.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone AN1-15
Immunohistochemistry was performed on normal biopsies of deparaffinized Human thyroid tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Anti-Androgen Receptor antibody [AN1-15] (ab2742) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-Androgen Receptor antibody [AR 441] - ab77557 from Abcam

Prices $373.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AR 441

Anti-AP2 alpha antibody - ChIP Grade - ab52222 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AP2 alpha antibody - ChIP Grade (ab52222) at 1/500 dilutionLane 1 : extracts from COLO205 cells,Lane 2 : extracts from COLO205 cells, with immunizing peptide

Anti-AP2 alpha antibody - ChIP Grade - ab52222 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AP2 alpha antibody - ChIP Grade (ab52222) at 1/500 dilutionLane 1 : extracts from COLO205 cells,Lane 2 : extracts from COLO205 cells, with immunizing peptide

Anti-Apolipoprotein B antibody - ab98132 from Abcam

Prices $378.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labelled with ab98132 at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Aryl hydrocarbon Receptor antibody - ab84833 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Aryl hydrocarbon Receptor antibody (ab84833) at 1/200 dilution + HepG2 cell lysate

Anti-beta Catenin antibody [15B8] - ab6301 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 15B8
All lanes : Anti-beta Catenin antibody [15B8] (ab6301) at 1 µg/mlLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-BMAL1 antibody - ChIP Grade - ab3350 from Abcam

Prices $391.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BMAL1 antibody - ChIP Grade (ab3350) at 1/500 dilutionLane 1 : U251 cell lysateLane 2 : U87-MG cell lysateLane 3 : NIH-3T3 cell lysateLysates/proteins at 25 µg per lane.

Anti-BRCA1 antibody [8F7] - ab94 from Abcam

Host Mouse
Clonality Monoclonal
Clone 8F7
IHC image of ab94 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Bub3 antibody - ab4180 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Bub3 antibody (ab4180) at 1/500 dilution + HeLa Nuclear Extract at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Anti-butyrate response factor 1 antibody - ab42473 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-butyrate response factor 1 antibody (ab42473) at 0.125 µg/ml (5% skimmed milk / PBS) + 10µg Human placenta whole cell lysateSecondaryHRP conjugated anti-rabbit at 1/50000 dilution

Anti-c-Fos antibody - ab429 from Abcam

Prices $378.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-c-Fos antibody (ab429) at 1/500 dilution + Whole cell lysate of chicken embryonic fibroblasts at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1000 dilutiondeveloped using the ECL technique

Anti-c-Maf antibody - ab427 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-c-Rel antibody - ab7556 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-c-Rel antibody - ab19464 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CD31 antibody [HEC7] - ab119339 from Abcam

Prices $391.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone HEC7
Immunofluorescent analysis of CD31 (green) showing staining in the membrane and cytoplasm of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD31 monoclonal antibody (ab119339) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-CEBP Alpha antibody [EP709Y] - ab40764 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP709Y
Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/20000 dilution + U937 cell lysate at 10 µg

Anti-CEBP Beta antibody [18F8] - ChIP Grade - ab15049 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 18F8

Anti-CEBP Beta antibody [E299] - ab32358 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E299
Overlay histogram showing HeLa cells stained with ab32358 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32358, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CPP Binding Protein antibody - ab28686 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
This image shows Rat brain tissue stained with ab28686.

Anti-CREB + CREM antibody - ab5803 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB polyclonal antibody (ab5803) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

Anti-CREB antibody - ab7540 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CREB antibody - ab7540 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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