ChIP-PCR

TRX2 Antibody - A300-113A from Bethyl Labs

Prices	$339.00
Sizes	100 µl (1 mg/ml)
Host	Rabbit
Clonality	Polyclonal

<b>Detection of Human Trithorax 2 by Western Blot and Immunoprecipitation.</b> <i>Samples</i>: A. Whole cell lysate (100 µg - E; 10 µg - T) from 293T cells that were untreated (E) or transfected with an expression construct containing the residues c-terminal to the consensus cleavage site within TRX2 (T). B. Whole cell lysate (100 µg - Input; one 10 cm plate for IP) from untreated 293T cells. <i>Antibodies</i>: Affinity purified rabbit anti-TRX2 antibody A300-113A used at the indicated concentrations for WB (A) and 10 µg/plate for IP (B). Immunoprecipitated TRX2 was detected by WB using A300-113A or rabbit anti-TRX2 antibody BL836. <i>Detection</i>: Chemiluminescence with exposure times of 10 (A) or 1 seconds (B). <br /> <br /> <br /> <br />

CTCF polyclonal antibody - Classic - C15410210 from Diagenode

Prices	$295.00
Sizes	50 μg/ 20 μl
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CTCF</strong><br />ChIP was performed with the Diagenode antibody against CTCF (Cat. No. C15410210) on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the Sat2 satellite repeat region, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ER alpha monoclonal antibody - Classic - C15100066 from Diagenode

Prices	$295.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal

<strong> Figure 1. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ERalpha </strong><br />ChIP was performed with the Diagenode monoclonal antibody against ERalpha (cat. No. AC-066-100) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The IP’d DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).

EZH2 polyclonal antibody - Classic - C15410039 from Diagenode

Prices	$295.00
Sizes	50 µg/50 µl
Host	Rabbit
Clonality	Polyclonal

<strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against EZH2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against EZH2 (Cat. No. C15410039) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

FOXA1 polyclonal antibody - Classic - C15410231-25 from Diagenode

Prices	$125.00
Sizes	25 μg
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

GR monoclonal antibody - Classic - C15200010 from Diagenode

Prices	$295.00
Sizes	50 µg/50 µl
Host	Mouse
Clonality	Monoclonal

<strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 μg of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR.

H2A.Z polyclonal antibody - Premium (sample size) - C15410201-10 from Diagenode

Prices	$80.00
Sizes	10 µg
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against H2A.Z</strong><br /> Figure 1A ChIP assays were performed using human HeLa cells, the Diagenode antibody against H2A.Z (cat. No. C15410201) and optimized PCR primer pairs for qPCR. ChIP was performed with the ““Auto Histone ChIP-seq” kit (cat. No. AB-Auto02-A100) on the IP-Star automated system, using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes c-fos and EIF2S3, used as positive controls, and for the inactive TSH2B gene and the Sat2 satellite repeat, used as negative controls. Figure 1B ChIP assays were performed using human K562 cells, the Diagenode antibody against H2A.Z (cat. No. C15410201) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP- seq” ki

H2A.Zac polyclonal antibody - Classic - C15410173 from Diagenode

Prices	$295.00
Sizes	50 μg/72 μl
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against H2A.Zac</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against H2A.Zac (Cat. No. pAb-173-050) and optimized primer pairs for qPCR. ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. AB-Auto02-A100) on the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/ IP) was used as negative IP control. QPCR was performed using primers specific for the promoters of the ACTB and EIF4A2 genes, used as positive control targets and for the coding region of the MYT1 gene, used as a negative control target. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H2A.Z is present at active promoters.

H2A.Zac polyclonal antibody - Premium - C15410202 from Diagenode

Prices	$375.00
Sizes	50 μg/46 μl
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against H2A.Zac</strong><br /> Figure 1A ChIP assays were performed using human HeLa cells, the Diagenode antibody against H2A.Zac (Cat. No. C15410202) and optimized PCR primer pairs for qPCR. ChIP was performed with the ““Auto Histone ChIP-seq” kit (Cat. No. AB-Auto02-A100) on the IP-Star automated system, using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Figure 1B ChIP assays were performed using human K562 cells, the Diagenode antibody against H2A.Zac (Cat. No. C15410202) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. AB-001-0024) on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. Quantitati

H2BK12ac polyclonal antibody - Classic (sample size) - C15410212-10 from Diagenode

Prices	$80.00
Sizes	10 μg
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against H2BK12ac</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H2BK12ac (Cat. No. C15410212) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010051), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

H2BK15ac polyclonal antibody - Classic (sample size) - C15410220-10 from Diagenode

Prices	$80.00
Sizes	10 μg
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against H2BK15ac</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H2BK15ac (Cat. No. C15410220) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010051), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

H3K18ac polyclonal antibody - Classic (sample size) - C15410139-10 from Diagenode

Prices	$80.00
Sizes	10 μg
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K18ac</strong><br /> ChIP assays were performed using human HeLa cells, treated with TSA, the Diagenode antibody against H3K18ac (cat. No. C15410139) and optimized PCR primer pairs for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. AB-001-0024), using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

H3K27ac polyclonal antibody - Classic - C15410174 from Diagenode

Prices	$295.00
Sizes	50 μg/42 μl
Host	Rabbit
Clonality	Polyclonal

<strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K27ac</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against H3K27ac (Cat. No. C15410174) and optimized PCR primer sets for qPCR. ChIP was performed with the “Auto Histone ChIP-seq” kit (Cat. No. C01010022), using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoter of the active ACTB (Cat. No. pp-1005-050) and EIF4A2 genes, used as positive controls, and for the coding region of the inactive MYT1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27 acetylation is enriched at the pr

H3K27ac polyclonal antibody - Classic (sample size) - C15410174-10 from Diagenode

Prices	$80.00
Sizes	10 μg
Host	Rabbit
Clonality	Polyclonal

H3K27ac polyclonal antibody - Premium - C15410196 from Diagenode

Prices	$375.00
Sizes	50 μg/18 μl
Host	Rabbit
Clonality	Polyclonal

<strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K27ac </strong><br />Figure 1A ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3K27ac (Cat. No. C15410196) and optimized PCR primer pairs for qPCR. ChIP was performed with the ““Auto Histone ChIP-seq” kit (cat. No. AB- Auto02-A100) on the IP-Star automated system, using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and ACTB genes, used as positive controls, and for the inactive TSH2B and MYT1 genes, used as negative controls.

H3K27ac polyclonal antibody - Premium (sample size) - C15410196-10 from Diagenode

Prices	$80.00
Sizes	10 µg
Host	Rabbit
Clonality	Polyclonal

H3K27me3 polyclonal antibody - Classic - C15410069 from Diagenode

Prices	$295.00
Sizes	50 μg/34 μl
Host	Rabbit
Clonality	Polyclonal

<strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K27me3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3K27me3 (Cat. No. C15410069) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010051), using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes EIF4A2 and GAPDH as negative controls, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3 is preferably present at inactive genes.

H3K27me3 polyclonal antibody - Premium - C15410195 from Diagenode

Prices	$375.00
Sizes	50 μg/27 μl
Host	Rabbit
Clonality	Polyclonal

H3K27me3 polyclonal antibody - Premium (sample size) - C15410195-10 from Diagenode

Prices	$80.00
Sizes	10 µg
Host	Rabbit
Clonality	Polyclonal

H3K36ac polyclonal antibody - Classic (sample size) - C15410307-10 from Diagenode

Prices	$80.00
Sizes	10 μg
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K36ac</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3K36ac (cat. No. C15410307) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the ACTB promoter and for the GAPDH promoter, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

H3K36me2 polyclonal antibody - Classic - C15310127 from Diagenode

Prices	$295.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K36me2</strong><br />ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3K36me2 (Cat. No. C15310127) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (Cat. No. C01010070), using sheared chromatin from 10,000 cells. A titration of the antibody consisting of 1, 5, and 10 μl per ChIP experiment was analysed. Additionally, the same titration was analysed after incubation of the antibody with 5 nmol blocking peptide (Cat. No. C16000127 ) for 1 hour at room temperature. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and ALDOA and for the coding region of the myogenic differentiation gene (MYOD). Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

H3K36me3 polyclonal antibody - Classic - C15310058 from Diagenode

Prices	$295.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3 </strong><br />ChIP was performed with 5 μl of the Diagenode antibody against H3K36me3 (cat. No. CS-058-050) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (cat. No. AB-Auto02-A100) on the IP-Star automated system. IgG (2 μg/IP) was used as a negative IP control. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the coding and promoter region of the active GAPDH gene, for the coding region of the inactive TSH2B gene and for the Sat2 satellite repeat (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2B shows the results in 200 kb regions of chromosome 12 (including the GAPDH positive control), 6 and 7

H3K36me3 polyclonal antibody - Classic - C15410058 from Diagenode

Prices	$295.00
Sizes	50 µg/56 µl
Host	Rabbit
Clonality	Polyclonal

<strong> Figure 1. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3</strong><br /> ChIP was performed with 2 μg of the Diagenode antibody against H3K36me3 (Cat. No. C15410058) on sheared chromatin from 1 million HeLaS3 cells using the “iDeal ChIP-seq” kit (Cat. No. C01010051). IgG (2 μg/IP) was used as a negative IP control. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the coding region of the active ACTB gene (figure 1A). The IP’d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1B shows the obtained profiles in genomic regions of chromosome 12 (including the GAPDH positive control), 7 (including the ACTB po

H3K36me3 polyclonal antibody - Classic (sample size) - C15410058-10 from Diagenode

Prices	$80.00
Sizes	10 µg
Host	Rabbit
Clonality	Polyclonal

H3K36me3 polyclonal antibody - Premium - C15410192 from Diagenode

Prices	$375.00
Sizes	50 μg/42 μl
Host	Rabbit
Clonality	Polyclonal

<strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K36me3</strong><br /> Figure 1A ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3K36me3 (Cat. No. C15410192) and optimized PCR primer pairs for qPCR. ChIP was performed with the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system, using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the promoter and a region located 1 kb upstream of the promoter of the GAPDH gene, used as negative controls. Figure 1B ChIP assays were performed using human K562 cells, the Diagenode antibody against H3K36me3 (Cat. No. C15410192) and optimized PCR primer pairs for qPCR. ChIP was performed with

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