RELA

Gene Symbol RELA
Entrez Gene 5970
Alt Symbol NFKB3, p65
Species Human
Gene Type protein-coding
Description v-rel avian reticuloendotheliosis viral oncogene homolog A
Other Description NF-kappa-B p65delta3|nuclear factor NF-kappa-B p65 subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|transcription factor p65|v-rel reticuloendotheliosis viral oncogene homolog A
Swissprots Q04206 Q6GTV1 Q6SLK1
Accessions AAR13863 CAA80524 CCO13802 EAW74424 EAW74425 EAW74426 Q04206 AK303262 BAG64344 AK308020 AK308478 BC011603 AAH11603 BC014095 AAH14095 BC033522 AAH33522 BC069248 BC110830 AAI10831 BM916512 BU633061 DA020095 L19067 AAA20946 M62399 AAA36408 U33838 AAA92888 U88316 AAB48487 XM_011545206 XP_011543508 XM_011545207 XP_011543509 NM_001145138 NP_001138610 NM_001243984 NP_001230913 NM_001243985 NP_001230914 NM_021975 NP_068810
Function NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with ot
Subcellular Location Nucleus. Cytoplasm. Note=Colocalized with DDX1 in the nucleus upon TNF-alpha induction (By similarity). Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation. {ECO:0000250}.
Top Pathways Sphingolipid signaling pathway, Apoptosis, Herpes simplex infection, Prolactin signaling pathway, Legionellosis

Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated) - 4025 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 93H1
Western blot analysis of NF-κB Control Cell Extracts #9243 (HeLa untreated or treated with hTNF-α #8902, 20 ng/ml, 5 min.), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated).

Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 4886 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 93H1
Flow cytometric analysis of HeLa cells, untreated (blue) or TNF-α-treated (green), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor ® 488 Conjugate).

Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 4887 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 93H1
Flow cytometric analysis of THP-1 cells, untreated (blue) or TNF-α treated (green), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 647 Conjugate).

Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate) - 5733 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 93H1
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with TNF-α #8902 and Calyculin A #9902 (green), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate).

NF-κB p65 (D14E12) XP ® Rabbit mAb (PE Conjugate) - 9609 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D14E12
Flow cytometric analysis of HeLa cells using NF-κB p65 (D14E12) XP ® Rabbit mAb (PE Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate) #5742 (red).

NF-κB p65 (L8F6) Mouse mAb (PE Conjugate) - 9460 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone L8F6
Flow cytometric analysis of HeLa cells using NF-κB p65 (L8F6) Mouse mAb (PE Conjugate) (green) compared to a non-specific isotype control.

Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb - 3033 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 93H1
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TNF-α treated (#2169, 20 ng/ml for 5 minutes), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (upper) or NF-κB p65 Antibody #3034 (lower).

Phospho-NF-κB p65 (Ser536) (7F1) Mouse mAb - 3036 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 7F1
Western blot analysis of extracts from HeLa cells, untreated or TNF-alpha-treated (#2169, 20ng/ml), using Phospho-NF-kappaB p65 (Ser536) (7F1) Mouse mAb (upper) or NF-kappaB p65 Antibody #3034 (lower).

Phospho-NF-κB p65 (Ser536) Antibody - 3031 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot anlaysis of extracts from HeLa cells, untreated or TNF-alpha-treated (#2169, 20 ng/ml) for the indicated times, using Phospho-NF-kappaB p65 (Ser536) Antibody (top) or NF-kappaB p65 Antibody #3034 (bottom).

Phospho-NF-κB p65 (Ser468) Antibody - 3039 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells treated for 5 minutes with TNF-alpha #2169 (20 ng/ml), Calyculin A #9902 (50 nM), or both compounds, using Phospho-NF-kappaB p65 (Ser468) Antibody (top) or NF-kappaB p65 Antibody #3034 (bottom).

Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb - 13346 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone E1Z1T
Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight), untreated (-) or LPS-treated (1 μg/ml, 15 min; +), using Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (upper) or NF-κB p65 (D14E12) XP ® Rabbit mAb #8242 (lower).

Acetyl-NF-κB p65 (Lys310) Antibody - 3045 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with NF-κB or NF-κB plus CBP, using Acetyl-NF-κB (Lys310) Antibody. Western blots were competed with acetylated peptide (middle) or non-acetylated peptide (right).

Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb - 12629 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2S3J
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged human NF-κB p65 (hNF-κB p65; +) and HA-tagged human p300 (hp300-HA; +), using Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb (upper) or NF-κB p65 (D14E12) XP ® Rabbit mAb #8242 (lower).

Methyl-NF-κB p65 (Lys310) Antibody - 13188 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human NF-κB p65 (hNF-κB p65-Myc/DDK; +) and Myc/DDK-tagged full-length human SETD6 (hSETD6-Myc/DDK; +), using Methyl-NF-κB p65 (Lys310) Antibody (upper) or DYKDDDDK Tag Antibody #2368 (lower).

NF-κB p65 (D14E12) XP ® Rabbit mAb - 8242 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D14E12
Western blot analysis of extracts from various cell lines using NF-κB p65 (D14E12) XP ® Rabbit mAb.

NF-κB p65 (C22B4) Rabbit mAb - 4764 from Cell Signaling Technology

Prices $99.00, $256.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C22B4
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (C22B4) Rabbit mAb (lower).

NF-κB p65 (L8F6) Mouse mAb - 6956 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L8F6
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® NF-κB p65 siRNA I #6261 (+), using NF-κB p65 (L8F6) Mouse mAb (upper) or α-Tubulin (11Η10) Rabbit mAb #2125 (lower). The NF-κB p65 (L8F6) Mouse mAb confirms silencing of NF-κB p65 expression, while the α-Tubulin (11Η10) Rabbit mAb is used as a loading control.

Phospho-NF-κB p65 (Ser276) Antibody - 3037 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with calyculin A #9902 (50 nM) for 10 minutes, using Phospho-NF-κB p65 (Ser276) Antibody (upper) or NF-κB p65 Antibody #3034 (lower).

NF-kappaB Antibody - 3032 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

NF-κB p65 Antibody - 3034 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot anlaysis of extracts from HeLa cells, untreated or TNF-α-treated (#2169, 20 ng/ml) for the indicated times, using Phospho-NF-κB p65 (Ser536) Antibody #3031 (top) or NF-κB p65 Antibody (bottom).

NF-κB p65 (E498) Antibody - 3987 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using NF-κB p65 (E498) Antibody.

NFκB p65 (C-20) - sc-372 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of NFκB p65 in NIH/3T3 (A,D), A-431 (B,E) and K-562 (C,F) whole cell lysates. Antibodies tested include NFκB p65 (C-20): sc-372 (A-C) and NFκB p65 (C-20)-G: sc-372-G (D-F).

NFκB p65 (F-6) - sc-8008 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NFκB p65 (F-6): sc-8008. Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

NFκB p65 (A) - sc-109 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Influence of okadaic acid (OA) and tumor necrosis factor (TNF) on the translocation of NFκB p65 from the cytoplasm to the nucleus. Cells were immunostained with NFκB p65 (A): sc-109 and visualized with biotinylated goat anti-rabbit IgG and fluorescein isothiocyanate-conjugated avidin. Kindly provided by Sree Devi Menon.

NFκB p65 (H-286) - sc-7151 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ChIP analysis of cofactor occupancy dynamics on the <i>IL</i>-<i>8</i> promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211, p300 (C-20): sc-sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek <i>et al</i>., Cell 2002, 110: 55-67.
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