SAE1

Gene Symbol SAE1
Entrez Gene 10055
Alt Symbol AOS1, HSPC140, SUA1, UBLE1A
Species Human
Gene Type protein-coding
Description SUMO1 activating enzyme subunit 1
Other Description SUMO-1 activating enzyme E1 N subunit|SUMO-1 activating enzyme subunit 1|SUMO-activating enzyme subunit 1|activator of SUMO1|sentrin/SUMO-activating protein AOS1|ubiquitin-like 1-activating enzyme E1A|ubiquitin-like protein SUMO-1 activating enzyme
Swissprots G3XAK6 Q9UBE0 O95717 B2RDP5 Q9P020 F5GXX7 B3KMQ2
Accessions EAW57460 EAW57461 EAW57462 EAW57463 EAW57464 Q9UBE0 AF046025 AAD23902 AF090385 AAD12785 AF110956 AAD24433 AF161489 AAF29104 AI339364 AK021978 BAG51064 AK027595 BAG51356 AK055481 AK090645 AK302287 BAG63628 AK315624 BAG37992 BC000344 AAH00344 BC003611 AAH03611 BC018271 AAH18271 BC128518 BC128519 BT007290 AAP35954 CN386158 DA047601 DA086973 DC297573 DC312737 DQ891751 ABM82677 DQ894932 ABM85858 XM_006722963 XP_006723026 XM_006722965 XP_006723028 XM_011526305 XP_011524607 NM_001145713 NP_001139185 NM_001145714 NP_001139186 NM_005500 NP_005491 NR_027280
Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. {ECO:0000269|PubMed:10187858, ECO:0000269|PubMed:10217437, ECO:0000269|PubMed:11451954, ECO:0000269|PubMed:11481243, ECO:0000269|PubMed:15660128, ECO:0000269|PubMed:20164921, ECO:0000269|PubMed:9920803}.
Subcellular Location Nucleus {ECO:0000269|PubMed:11481243}.
Tissue Specificity Expression level increases during S phase and drops in G2 phase (at protein level). {ECO:0000269|PubMed:11481243}.
Top Pathways Ubiquitin mediated proteolysis

UBLE1A/SAE1 Antibody - 13585 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using UBLE1A/SAE1 Antibody.

AOS-1 (H-9) - sc-398080 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AOS-1 (H-9): sc-398080. Western blot analysis of AOS-1 expression in MCF7 (A), T-47D (B), SW480 (C), HeLa (D) and NIH/3T3 (E) whole cell lysates.

AOS-1 (C-14) - sc-46763 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AOS-1 (C-14): sc-46763. Western blot analysis of AOS-1 expression in non-transfected: sc-110760 (A) and human AOS-1 transfected: sc-112313 (B) 293 whole cell lysates.

AOS-1 (C-15) - sc-46764 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AOS-1 (C-15): sc-46764. Western blot analysis of AOS-1 expression in non-transfected: sc-110760 (A) and human AOS-1 transfected: sc-112313 (B) 293 whole cell lysates.

AOS-1 (E-12) - sc-376628 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AOS-1 (E-12): sc-376628. Western blot analysis of AOS-1 expression in non-transfected: sc-117752 (A) and human AOS-1 transfected: sc-176690 (B) 293T whole cell lysates.

AOS-1 (G-3) - sc-376146 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AOS-1 (G-3): sc-376146. Western blot analysis of AOS-1 expression in non-transfected: sc-117752 (A) and human AOS-1 transfected: sc-176690 (B) 293T whole cell lysates.

AOS-1 (H-7) - sc-271592 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AOS-1 (H-7): sc-271592. Western blot analysis of AOS-1 expression in non-transfected: sc-110760 (A) and human AOS-1 transfected: sc-112313 (B) 293 whole cell lysates.

AOS-1 (N-14) - sc-46765 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AOS-1 (N-14): sc-46765. Western blot analysis of AOS-1 expression in SW480 (A), HeLa (B), NIH/3T3 (C), MCF7 (D), T-47D (E) and PC-12 (F) whole cell lysates.

AOS-1 (N-18) - sc-46766 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AOS-1 (N-18): sc-46766. Western blot analysis of AOS-1 expression in SW480 (A), HeLa (B), NIH/3T3 (C), MCF7 (D), T-47D (E) and PC-12 (F) whole cell lysates.

Anti-SAE1 antibody [1G8] - ab140964 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G8
All lanes : Anti-SAE1 antibody [1G8] (ab140964) at 1/4000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY SAE1 cDNALysates/proteins at 5 µg per lane.

Anti-SAE1 antibody [2946C4a] - ab71959 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2946C4a
Anti-SAE1 antibody [2946C4a] (ab71959) + immunising recombinant protein

Anti-SAE1 antibody [EPR15397(B)] - ab185949 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15397(B)
All lanes : Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilutionLane 1 : Hela cell lysateLane 2 : Jurkat cell lysateLane 3 : MCF7 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-SAE1 antibody [EPR15398] - ab185552 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15398
Immunoprecipitation from 293 cells using ab185552 at 1/50 dilution.  Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.  Blocking buffer: 5% NFDM/TBST.

Anti-SAE1 antibody - ab58383 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SAE1 antibody (ab58383) at 1/500 dilutionLane 1 : 293 cell extractLane 2 : 293 cell extract with immunising peptide

Anti-SAE1 antibody - ab38434 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SAE1 antibody (ab38434) + Mouse heart tissue lysate

Anti-SAE1 antibody - ab56957 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
SAE1 antibody (ab56957) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human adrenal gland.

Anti-SAE1 antibody - ab5645 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-SAE1 Antibody (clone 6C7) - LS-C339476 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6C7
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-SAE1 Antibody (clone 3C3) - LS-C339550 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C3
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-SAE1 Antibody (clone 6B11) - LS-C339477 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6B11
IHC of paraffin-embedded Human endometrium tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-SAE1 Antibody - LS-C17948 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Sheep
Clonality Polyclonal

Anti-SAE1 Antibody - LS-C76152 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-SAE1 Antibody - LS-C122457 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-SAE1 Antibody - LS-C132310 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SAE1 Antibody - LS-C147913 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
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