| Gene Symbol | Nol3 |
|---|---|
| Entrez Gene | 78688 |
| Alt Symbol | ARC, B430311C09Rik, MYC, NOP, Nop30 |
| Species | Mouse |
| Gene Type | protein-coding |
| Description | nucleolar protein 3 (apoptosis repressor with CARD domain) |
| Other Description | apoptosis repressor with CARD|nucleolar protein 3 |
| Swissprots | Q9D1X0 |
| Accessions | EDL11261 Q9D1X0 AK021023 BAB32281 AK053846 BAC35554 AK079536 BAC37674 AK197583 AY459322 AAR22550 BC027290 AAH27290 BC053971 XM_006531503 XP_006531566 NM_030152 NP_084428 |
| Function | Apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly (By similarity). Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation (By similarity). Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors (PubMed:16505176) (PubMed:24312627). Inhibits calcium-mediated cell death by functionning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis (By similarity). Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mito |
| Subcellular Location | Cytoplasm {ECO:0000250|UniProtKB:O60936, ECO:0000250|UniProtKB:Q62881}. Mitochondrion {ECO:0000250|UniProtKB:Q62881}. Sarcoplasmic reticulum {ECO:0000250|UniProtKB:Q62881}. Note=Phosphorylation at Thr-149 results in translocation to mithochondria. Colocalized with mitochondria in response to oxidative stress. {ECO:0000250|UniProtKB:Q62881}. |
ARC CRISPR/Cas9 KO Plasmid (m) - sc-429694 from Santa Cruz Biotechnology
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ARC HDR Plasmid (m) - sc-429694-HDR from Santa Cruz Biotechnology
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ARC Double Nickase Plasmid (m) - sc-429694-NIC from Santa Cruz Biotechnology
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ARC Double Nickase Plasmid (m2) - sc-429694-NIC-2 from Santa Cruz Biotechnology
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ARC CRISPR Activation Plasmid (m) - sc-429694-ACT from Santa Cruz Biotechnology
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ARC CRISPR Activation Plasmid (m2) - sc-429694-ACT-2 from Santa Cruz Biotechnology
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ARC Lentiviral Activation Particles (m) - sc-429694-LAC from Santa Cruz Biotechnology
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ARC Lentiviral Activation Particles (m2) - sc-429694-LAC-2 from Santa Cruz Biotechnology
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ARC siRNA (m) - sc-29723 from Santa Cruz Biotechnology
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ARC shRNA Plasmid (m) - sc-29723-SH from Santa Cruz Biotechnology
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ARC shRNA (m) Lentiviral Particles - sc-29723-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU170301 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN36558 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND36558 from Sigma-Aldrich
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NOL3 siRNA (Mouse) - CRM9953 from Cohesion Biosciences
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shRNA set against Mouse NOL3 (NM_030152.4) - SHH353540 from Creative biogene
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shRNA set against Mouse Nol3(NM_030152.4) - SHR015576 from Creative biogene
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Mouse NOL3 siRNA - orb289429 from Biorbyt
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NOL3 shRNA Plasmid (Mouse) - CHM9953 from Cohesion Biosciences
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NOL3 miRNA 3'UTR clone - MiUTR1M-07732 from Creative biogene
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